Fig. 2From: Expression of FGD4 positively correlates with the aggressive phenotype of prostate cancerInhibition of FGD4 expression upon FGD4 siRNA transfection of prostate cancer cells. a and d: Quantitative real-time PCR analysis of FGD4 mRNA in siRNA or control RNA transfected PC-3 and LNCaP-104S cells. Raw data have been normalized to the average of RPL13A and GAPDH, two control genes. Data show at least 70% reduction in FGD4 mRNA by FGD4 siRNAs compared to control siRNAs. b and e: Immunoblot analysis of PC-3 and LNCaP-104S cell lysates transfected with FGD4 siRNAs showing substantial reduction in FGD4 upon siRNA transfection. Alpha-tubulin and GAPDH were used as the loading controls c and f: Densitometric analyses of protein concentration normalized to the internal controls.Back to article page