Fig. 2

TH588 and IR induce cell death in HCT116. For all experiments with chemical agents control cells were treated with vehicle only (DMSO). Cells were irradiated and immediately treated afterwards and then incubated for 48 h. a After staining with PI (red) and Hoechst 33342 (blue) immunofluorescence images were recorded. b Dead cells and total cells were counted. The ratio of dead divided by total cells is displayed. Etoposide was used as a positive control. Bonferroni’s test was used as post-hoc test. c Colony formation assays were performed in collagen coated dishes. After irradiation cells were treated with TH588 and incubated for 10 days. Only colonies with more than 50 cells were counted. Bonferroni’s post-hoc test was used to determine significant differences between groups