Fig. 1

Dose-dependent decrease of viability in cancer and non-cancer cell lines after TH588 treatment. HCT116, SW480 and HUVEC were plated on 96-well-plates. Incubations took place for 72 h. MTT reagent was added 4 h before lysis of the cells. Viability was assessed by means of an MTT assay. Values are given as % of the untreated control in each group. Bonferroni’s post-hoc test was used to determine significant differences between groups. a Viability of different cell lines after TH588-treatment. HUVEC were in a proliferating state when 8.000 cells per well had been plated. 40.000 HUVEC per well led to a confluent cell layer. Cells were treated with final concentrations of 5 or 10 μM TH588 (b) Viability of cells treated with 5 μM TH588, doxycycline for knockdown induction, or both