Fig. 5

EMX2 overexpression strongly diminished S phase of the cell cycle in U87 cells. Cell number distribution among cell cycles phases was measured for three empty clones (Control) and six distinct EMX2 clones (EMX2 cl.A.1, EMX2 cl.A.2 and EMX2 cl.A.3, EMX2 cl.B.1, EMX2 cl.B.2 and EMX2 cl.B.3). Cells were cultured without (No Tet) or with tetracycline for 2 days (D2 Tet), 6 days (D6 Tet), or 8 days followed by 8 days of culture without tetracycline (D8 Tet). After treatment, cells were stained with propidium iodide then analyzed by a FACscan flow cytometer. The peaks in the illustration correspond to the G0/G1, S and G2/M phases (a). Percentages in each phase of the cell cycle were automatically measured by Modfit LT. A representative histogram of cell cycle distribution is shown in b. Data are expressed as mean ± SE. The No Tet condition was used as reference in the t-tests and p-values were adjusted with BH correction * p < 0.05, ** p < 0,01