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Fig. 2 | BMC Cancer

Fig. 2

From: The expression of EMX2 lead to cell cycle arrest in glioblastoma cell line

Fig. 2

EMX2 expression in GB patients and cell lines. a-b- Intratumor EMX2 expression in patients. EMX2 mRNA level was measured at four different GB zones from 10 patients (PBZ: peripheral brain zone, I: interface zone, TZ: tumor zone, and NZ: necrotic zone; [10]) and on two normal brain used as control (b: normal brain). Box plots representing mRNA expression levels obtained from microarray experiments for the EMX2 A_23_P44246 Agilent probe (a). p = 1.573e-06. EMX2 mRNA level was measured by RT-qPCR. Box plots representing fold changes calculated using RNA brain as sample reference and GAPDH and TBP as gene reference (b). p = 1.38e-02. c- EMX2 expression level in GB cell lines. RT-qPCR was used to measure EMX2 mRNA level in ten primary adherent cell cultures (1–10), five neurosphere cultures (a-d) and two immortalized GB cell lines U87 and U251. Relative EMX2 expression level was normalized to GAPDH and TBP reference genes and to a RNA brain reference sample. Experiments were performed in triplicate. Since relative EMX2 expression level was extremely low or null, the standard deviations could not be indicated in this table

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