Skip to main content
Fig. 2 | BMC Cancer

Fig. 2

From: The expression of EMX2 lead to cell cycle arrest in glioblastoma cell line

Fig. 2

EMX2 expression in GB patients and cell lines. a-b- Intratumor EMX2 expression in patients. EMX2 mRNA level was measured at four different GB zones from 10 patients (PBZ: peripheral brain zone, I: interface zone, TZ: tumor zone, and NZ: necrotic zone; [10]) and on two normal brain used as control (b: normal brain). Box plots representing mRNA expression levels obtained from microarray experiments for the EMX2 A_23_P44246 Agilent probe (a). p = 1.573e-06. EMX2 mRNA level was measured by RT-qPCR. Box plots representing fold changes calculated using RNA brain as sample reference and GAPDH and TBP as gene reference (b). p = 1.38e-02. c- EMX2 expression level in GB cell lines. RT-qPCR was used to measure EMX2 mRNA level in ten primary adherent cell cultures (1–10), five neurosphere cultures (a-d) and two immortalized GB cell lines U87 and U251. Relative EMX2 expression level was normalized to GAPDH and TBP reference genes and to a RNA brain reference sample. Experiments were performed in triplicate. Since relative EMX2 expression level was extremely low or null, the standard deviations could not be indicated in this table

Back to article page
\