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Fig. 1 | BMC Cancer

Fig. 1

From: Microenvironment in neuroblastoma: isolation and characterization of tumor-derived mesenchymal stromal cells

Fig. 1

Characterization of ex-vivo expanded NB-MSCs. a morphology of culture-expanded MSCs obtained from representative NB-MSC and BM-MSC cultures. MSCs from both the patient and donor display the characteristic spindle-shaped morphology. Original magnification × 4. b Cumulative PDs from P1 to P5 of MSCs isolated from BM (black line) and from NB tumor tissue (grey line). The data represent the mean (±SD) of seven NB-MSC and eight BM-MSC cultures. c Mean passages (±SD) at which BM-MSC (black bar) and NB-MSC (grey bar) entered senescence. d Osteogenic, adipogenic and chondrogenic differentiation capacity of MSCs: the differentiation into osteoblasts is demonstrated by the histological detection of ALP activity (I) and by the histological detection of calcium depositions positive for Alizarin Red S (II); the differentiation into adipocytes is revealed by the formation of lipid droplets stained with oil red O (III); the differentiation into chondrocytes is shown by alcian blue staining (IV). Original magnification 20x

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