Table 2 Limit of detection, sensitivity, specificity and accuracy of ALK fusion detection with massive parallel sequencing (IonTorrent) and a probe-based assay (NanoString) determined using a cell line dilution series
From: RNA-based analysis of ALK fusions in non-small cell lung cancer cases showing IHC/FISH discordance
| Â | Massive Parallel Sequencing (MPS) | Probe-Based Assay | ||
|---|---|---|---|---|
ALK 3′/5’ Imbalance | ALK Fusion | ALK 3′/5’ Imbalance | ALK Fusion | |
H2228 Cell Line Dilution in Palatine Tonsil Background | ||||
 100% | Uncertain | Yes | Yes | Yes |
 50% | Uncertain | Yes | Yes | Yes |
 30% | Uncertain | Yes | Yes | Yes |
 10% | Uncertain | Yes | Yes | No |
 5% | No | No | Yes | No |
 1% | No | No | No | No |
 0% | No | No | No | No |
Assay Parameter | ||||
 Sensitivity | 33.3% | 66.7% | 83.3% | 50.0% |
 Specificity | 100% | 100% | 100% | 100% |
 NPV | 20.0% | 33.3% | 50.0% | 25.0% |
 PPV | 100% | 100% | 100% | 100% |
 Accuracy | 42.9% | 71.4% | 85.7% | 57.1% |