Skip to main content

Table 2 Limit of detection, sensitivity, specificity and accuracy of ALK fusion detection with massive parallel sequencing (IonTorrent) and a probe-based assay (NanoString) determined using a cell line dilution series

From: RNA-based analysis of ALK fusions in non-small cell lung cancer cases showing IHC/FISH discordance

  Massive Parallel Sequencing (MPS) Probe-Based Assay
ALK 3′/5’ Imbalance ALK Fusion ALK 3′/5’ Imbalance ALK Fusion
H2228 Cell Line Dilution in Palatine Tonsil Background
 100% Uncertain Yes Yes Yes
 50% Uncertain Yes Yes Yes
 30% Uncertain Yes Yes Yes
 10% Uncertain Yes Yes No
 5% No No Yes No
 1% No No No No
 0% No No No No
Assay Parameter
 Sensitivity 33.3% 66.7% 83.3% 50.0%
 Specificity 100% 100% 100% 100%
 NPV 20.0% 33.3% 50.0% 25.0%
 PPV 100% 100% 100% 100%
 Accuracy 42.9% 71.4% 85.7% 57.1%
  1. NPV negative predictive value, PPV positive predictive value