Fig. 1
From: RNA-based analysis of ALK fusions in non-small cell lung cancer cases showing IHC/FISH discordance
Samples tested for ALK fusions on protein, DNA and RNA level. Sample P19 shows ALK IHC uncertain and FISH-BL-positive results, whereas results on transcriptional level were negative. P15 is concordantly positive with all applied methods. P32 and P31 are both ALK IHC-negative/ FISH-positive. These samples are ALK fusion negative on transcriptional level with both RNA-based methods (NanoString and IonTorrent), whereas P32 shows no RNA count, indicating no ALK expression. HE: hematoxylin and eosin staining; IHC: immunohistochemistry; FISH: fluorescence in-situ hybridization: MPS: Massive Parallel Sequencing; NEG CTRLs: negative controls; Probe-assay data shown are absolute raw data counts