Fig. 7

Association between ALDH7A1 protein levels and patient survival in liver and kidney cancer. a-b Representative images of hepatocellular carcinoma (HCC) and renal clear cell carcinoma (ccRCC) patient biopsies stained with anti-ALDH7A1. Scale bar: 100 μm. Upper panel adjacent normal tissue and lower panel corresponding tumor tissue from the same patient. c-d 120 hepatocellular carcinoma and 149 renal clear cell carcinoma tissue and paired adjacent normal tissue were labeled with anti-ALDH7A1 and scored for antigen expression. The staining score shown was calculated by subtracting the normal tissue score from the corresponding tumor score. The p-value was determined by the Wilcoxon matched-pairs signed rank test. e-f Forest plot representing multivariate Cox proportional hazards regression models of the hepatocellular carcinoma and renal clear cell carcinoma data scored in (c-d). For each variable the square and horizontal lines represent the estimated Hazard Ratio (HR) and corresponding confidence interval, respectively. The value of the model was estimated by the likelihood ratio test, and different models were compared by chi-square test. g, h Kaplan-Meier plots to visualize the association shown in (e) and (f). Survival plots show early and late stage patient groups divided by the ALDH7A1 immunohistochemistry score. The p-values were determined by likelihood ratio testing