Fig. 2

Overexpression of Kpnβ1 results in reduced proliferation of cervical cancer and normal epithelial cells. a, b Trypan Blue assays revealed a significant reduction in the number of live HeLa (a) and CaSki (b) cells expressing Kpnβ1-EGFP compared to EGFP. Cells were counted for a period of up to 4 days post plating. c, d MTT cell proliferation assays reveal a significant reduction in the anchorage-independent proliferation of HeLa (c) and CaSki (d) cells stably overexpressing Kpnβ1. Cells growing adherently were plated in 1% methylcellulose-containing media into 96-well plates coated in 12 mg/ml poly-HEMA. Cell proliferation was assayed by the addition of the MTT reagent for a period of up to 8 days. Results shown represent the mean ± SEM (*p < 0.05). e Western blot analysis reveals moderate Kpnβ1 overexpression in ARPE-19 and hTERT-RPE-1 normal epithelial cell lines. f Trypan blue assays show a significant reduction in ARPT-19 and hTERT-RPE-1 cell number 96 h after transient Kpnβ1 overexpression. Results shown represent the mean ± SEM (*p < 0.05)