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Fig. 8 | BMC Cancer

Fig. 8

From: Protective role of all-trans retinoic acid (ATRA) against hypoxia-induced malignant potential of non-invasive breast tumor derived cells

Fig. 8

Role of ATRA in modulating PLC-β2 expression in MCF10DCIS cells. a Quantitative RT-PCR analysis of PLCβ2 mRNA in MCF10DCIS cells grown for 96 h at normoxia or hypoxia in the presence or absence of 1 μM ATRA. Relative transcript levels were determined using the 2-Ct method and normalized to RPL13A mRNA. Values represent the fold changes ±SD relative to normoxia, taken as 1. b Representative fluorescence microscopy images of MCF10DCIS cells grown on glass dishes for 96 h at normoxia or hypoxia in the presence or absence of 1 μM ATRA and subjected to immunocytochemical analysis with the anti-PLC-β2 antibody. Bar: 20 μm. On the right, fluorescence intensity of digitized images calculated by the ImageJ software. c Cytofluorimetrical analysis of CD133 expression in MCF10DCIS cells transfected with siRNAs specific for PLC-β2 (PLC-β2 siRNAs) and cultured for 96 h under hypoxia in the presence or absence of 1 μM ATRA. Scramble siRNAs (Ctrl siRNAs) was used as control. Values represent the fold changes of the percentage of cells expressing high levels of CD133 ± SD relative to normoxia, taken as 1. All the data are the mean of three separate experiments performed in triplicate ±SD. #P < 0.05 versus normoxia; *P < 0.05 between bars

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