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Fig. 4 | BMC Cancer

Fig. 4

From: Stromal fibroblast activation protein alpha promotes gastric cancer progression via epithelial-mesenchymal transition through Wnt/ β-catenin pathway

Fig. 4

FAP promotes EMT of GC cells through Wnt/β-catenin pathway. Exogenous FAP promotes SGC EMT in dose-dependent manner. The expression of E-cadherin and ZO-1 were reduced, while that of N-cadherin and Vimentin were increased by qRT-PCR assay (a), and Western blotting assay (b). In addition, the DKK1 and LEF-1 protein, which could be participated in Wnt/β-catenin pathway, were also increased with more exogenous FAP. The result also accompanied in SGC cells co-cultured in SN and SF groups. c The morphology of SGC cells in SF group tended to be fibroblast-like, long fusiform, which was indicated by red arrows. d The expression of E-cadherin and ZO-1 were reduced, while that of N-cadherin and Vimentin were increased in SGC cells of SF group by Western blotting assay. Similarly, the DKK1 and LEF-1 protein were also increased. e The expression of E-cadherin was reduced, while that of α-SMA was increased both in GES1 cells (gastric normal cells) and SGC cells of SF group by the immunofluorescence staining

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