Fig. 5

Genetic inhibition of MCL-1 or overexpression of NOXA renders cells significantly more sensitive to bortezomib. a SU-DHL-4 cells were transfected with shRNA constructs designed against MCL-1. Two clones of shRNA MCL-1 were selected. These 2 clones of SU-DHL-4/shMCL1 and shControl cells were exposed to bortezomib (4 nM) for 48 h after which cells were analyzed by flow cytometry and western blot. b Similarly, SU-DHL-16 cells were transfected with shRNA constructs designed against MCL-1. SU-DHL-16/shMCL1 and shControl cells were exposed to bortezomib (1.5 nM) for 48 h after which cells were analyzed flow cytometry and Western blot. c SU-DHL-16 cells were transfected with NOXA/Flag constructs. Three clones with overexpression of NOXA were selected. These clones were exposed to HHT (10 nM) for 48 h then analyzed by flow cytometry. d SU-DHL-16 cells were transfected with shNOXA constructs. Two clones with knockdown of NOXA were selected. These clones were exposed to HHT (10 nM) for 48 h then analyzed by flow cytometry