Fig. 1

Reduced expression of EpCAM in PC-3-EpCAM-KD cell line. The knock-down effect of EpCAM was confirmed by Western blotting, immunofluorescence staining and qRT-PCR in PC-3 CaP cell line. a Western blotting is shown the reduced expression of EpCAM in PC-3-EpCAM-KD cell line compared with PC-3-EpCAM-scr and PC-3 wildtype cell lines. ß-tubulin was chosen as a loading control. b Representative immunofluorescence images of PC-3-EpCAM-KD, PC-3-EpCAM-scr and PC-3 wildtype cell lines. Green indicates positive-EpCAM staining and nuclei are stained with PI (red). Magnification: all images × 400. c Knock-down effects from Western blotting and immunofluorescence are further confirmed by qRT-PCR (♦ indicates P < 0.05). All results were from three independent experiments (mean ± SD, n = 3). knock-down; scr, scrambled shRNA control