Fig. 1
From: MiR-155, a potential serum marker of extramammary Paget’s disease
EMPD’s procedure chart and result of miRNA array. a Flow diagram of how our serum miRNA array was performed. b MiRNA expression profiles. MiRNA expressions of two pairs of normal and healthy volunteers and EMPD patients were profiled using TaqMan Human MicroRNA array card A (v2.1). The cycle threshold (Ct) values were obtained with SDS 2.3 and RQ manager 1.2 software (Applied Biosystem) and analyzed with RealTime StatMiner® 4.2 software (Integromics, Inc.). The -ΔCt was calculated and heat map analysis was performed with Excel. A green-yellow-red color scale (− 6.0 to 12.7) depicts normalized miRNAs expression level based on global normalization. c Differently expressed miRNAs between normal and EMPD, using -ΔCt from 2 decreased miRNAs (miR-122, miR-375) and 3 most significantly increased miRNAs (miR-155, miR-495, miR-652)