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Fig. 1 | BMC Cancer

Fig. 1

From: Exosomal miR-99a-5p is elevated in sera of ovarian cancer patients and promotes cancer cell invasion by increasing fibronectin and vitronectin expression in neighboring peritoneal mesothelial cells

Fig. 1

Exosomes were isolated from epithelial ovarian cancer (EOC) cell lines and then exosomal miRNAs were analyzed. a Electron microscopy. Exosomes were immunogold-labeled with anti-CD63 antibody. Transmission electron micrographs of purified exosomes secreted from several EOC cell lines are shown. Immortalized ovarian surface epithelium (IOSE) cells were used as a non-malignant control. Scale bar, 100 nm. Representative images are shown. b Nanoparticle tracking analysis. Concentration and size distribution of nano-sized particles in the exosome suspension were measured using a NanoSight system. The concentration presents the number of nano-sized particles per 1 mL culture medium. c RNA analyses. RNA was extracted from EOC-derived exosomes and analyzed using an Agilent 2100 Bioanalyzer (left). A profile of cellular RNA is shown as a control (right). Arrows indicate the position of small RNA and the 18S and 28S ribosomal RNAs. d Summary of exosomal miRNA microarray. Exosomal miRNAs that were upregulated by more than 3-fold in both EOC cells (HeyA8 and TYK-nu) vs. IOSE cells are listed. e miRNA qRT-PCR. Relative expression levels of miR-99a-5p in EOC-derived exosomes are shown. IOSE-derived exosomes were used as control. Data represent the mean ± standard deviation (SD) of three experiments. ***p < 0.001

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BMC Cancer

ISSN: 1471-2407

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