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Fig. 3 | BMC Cancer

Fig. 3

From: The small organic molecule C19 binds and strengthens the KRAS4b-PDEδ complex and inhibits growth of colorectal cancer cells in vitro and in vivo

Fig. 3

C19 induces apoptosis in colorectal cancer cells. a Cells were treated with the IC50 of C19 for 24, 48 and 72 h and cell lysates were analyzed for cleaved PARP. β-actin served as loading control. Representative blots of three independent experiments are shown. b Densitometric analysis of c-PARP normalized to total PARP levels in HCT116 and LoVo cells treated with C19 for 72 h. Error bars represent SEM. *p < 0.01 (c) Annexin V and PI analysis of HCT116 and LoVo cells treated with the IC50 concentration of C19 or vehicle alone for 72 h. C19 treatment strongly increased the percentages of LoVo cancer cells in early (Annexin V+ PI-) and late apoptosis (Annexin V+ PI+)

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