Table 1 RT-PCR primer sequences used in microRNAs validation
microRNAs | primer sequences |
|---|---|
miR-142-5p RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAGTAGTGC |
miR-142-5p F | ACACTCCAGCTGGGcataaagtagaaagcac |
miR-142-3p RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCCATAAA |
miR-142-3p F | ACACTCCAGCTGGGtgtagtgtttcctacttta |
miR-338-3p RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCAACAAAA |
miR-338-3p F | ACACTCCAGCTGGGtccagcatcagtgatttt |
miR-340-5p RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAATCAGTC |
miR-340-5p F | ACACTCCAGCTGGGttataaagcaatgagact |
miR-16-2-3p RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTAAAGCAG |
miR-16-2-3p F | ACACTCCAGCTGGGccaatattactgtgctgc |
hsa-miR-582-5p RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAGTAACTG |
hsa-miR-582-5p F | ACACTCCAGCTGGGttacagttgttcaaccagt |
hsa-u6 | CTCGCTTCGGCAGCACA |
hsa-u6 | AACGCTTCACGAATTTGCGT |