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Fig. 7 | BMC Cancer

Fig. 7

From: Analysis of oncogenic activities of protein kinase D1 in head and neck squamous cell carcinoma

Fig. 7

PKD1 overexpression promoted bombesin-stimulated proliferation of HNSCC cells. a and b Induced PKD1 expression promoted GRP or bombesin -induced ERK1/2 activation in HNSCC cells. Dox-inducible PKD1 overexpressing 686LN cell line was treated with or without Dox at 50 ng/ml for 24 h, followed by GRP or bombesin at 400 nM for the indicated times. The cells were harvested and subjected to Western blotting analysis. Representative images from one of four independent experiments are shown. c Induced PKD1 expression promoted bombesin-stimulated proliferation of 686LN cells. PKD1-inducible 686LN cells were pre-treated with 50 ng/ml Dox for 2 days to induce PKD1 expression. Cells with or without induced PKD1 were replated for proliferation assay, followed by treatment with or without bombesin. Cell proliferation in the absence of induced PKD1 (−Dox) treated with or without bombesin was plotted as control (inset). Representative data from one of three independent experiments are shown. * p < 0.05, ** P < 0.01, ***P < 0.001

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