Fig. 6

Effects of PKD1 on cell migration and invasion. a Wound healing assay. Confluent control (Con-c9), PKD1 (PKD1-c1 and PKD1-c45), and constitutive-active PKD1 (PKD1-CA-c37) expression clones were pre-treated with 500 ng/ml Dox for two days. Monolayers were wounded and imaged immediately (0 h). Wound closure was recorded after 12 h. The width of the wound is the average of 9 determinations per time point. Percent wound healing was calculated at each time point as described in “Materials and Methods”. b Matrigel invasion assay. A fixed number of control (Con-c9), PKD1 (PKD1-c1), and constitutive-active PKD1 (PKD1-CA-c37) stable expression cell lines (1.0 × 10⁵/ml) were seeded into the upper control or invasion chamber. After 22 h, non-invading cells were removed and cells that invaded through the matrigel were fixed, stained, and photographed under a microscope. Magnification, 200x. Percent invasion is expressed as the number of cells that invaded through the matrigel matrix relative to the number of cells that migrated through the control insert. Cell number is determined by counting total cell number in random 10 fields. The experiment was repeated thrice and a representative one is shown. ** P < 0.01