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Fig. 5 | BMC Cancer

Fig. 5

From: Analysis of oncogenic activities of protein kinase D1 in head and neck squamous cell carcinoma

Fig. 5

Induced PKD1 promoted the growth of HNSCC tumor xenografts. Control (Con-c9) and PKD1 (PKD1-c1) stable inducible clones were injected subcutaneously in both flanks of female nude mice. Once tumor becomes palpable, mice were randomized into four groups (Con-c9/−Dox, Con-c9/+Dox, PKD1-c1/−Dox, PKD1-c1/+Dox) with Con-c9/+Dox and PKD1-c1/+Dox receiving Dox at 1 mg/ml via drinking water. Tumor volume (a) and mouse weight (b) were measured every 2–3 days. At the end of the experiment, mice were sacrificed, tumors were dissected and a final tumor weight was recorded for each tumor (c). Representative images of mice with their respective excised tumors are shown (d). Statistical significance between treatment groups was determined using the Mann-Whitney-Wilcoxon test. ns, p ≥ 0.05. Tumor tissues were analyzed by Western blotting for PKD1 and downstream signaling pathways (e) and stained for Ki67 (f) by IHC and the staining was quantified (g). IHC images, × 200. **p < 0.01 by unpaired t-test

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