Fig. 5
Effect of TAX on protein expression of PI3K and phosphorylation of Akt at Ser473 in HCT116 and HT29 cells. Also the effect of treatment of TAX on HCT116 and HT29 cell lines on protein expression of Bax, Bcl2, Bak, Bcl-XL and PARP. Total cell lysate were prepared and 40 μg of protein was subjected to SDS-page followed by Immunoblot analysis and chemiluminescence detection. Equal loading of protein was confirmed by stripping the immunoblot and reprobing it for Actin
