Fig. 4

MALAT-1 upregulates ZEB1 expression by sponging miR-200c-3p. a The protein levels of ZEB1 were detected in response to transfection of miR-200c-3p nc, inhibitors, or mimics. The result showed that transfection of miR-200c-3p inhibitor triggered significant endogenous ZEB1 expression compared to that of nc in AsPC-1 and CFPAC-1 cells, whereas miR-200c-3p mimic silenced ZEB1 protein expression. b Western blot was performed to examine ZEB1 protein expression in Aspc-1 and CFPAC-1 after co-transfection with MALAT-1 nc/si1 or miR-200c-3p nc/inhibitor. Cotransfection with MALAT-1 siRNA and miR-200c-3p inhibitor showed that the miR-200c-3p inhibitor weakened the down-regulation of ZEB1 by MALAT-1 knockdown. β-actin was used as an internal control for protein loading. Relative densities are presented as the fold change relative to the internal control