Fig. 4From: Discovery of a diagnostic biomarker for colon cancer through proteomic profiling of small extracellular vesiclesImmunoblot showing candidate proteins from small-EVs and cell lysates of colon cancer and normal cells. The expression of selected proteins, namely TSPAN1, LGALS3BP, SLC1A5, CLDN7, and GPRC5A, in small-EVs was validated. Protein samples were prepared from small-EVs (10 μg protein) (a) and cell lysates (10 μg protein) (b) of HT-29, HCT-116, and CRL-1541 cell linesBack to article page