Fig. 2
Characterization of small-EVs isolated from HT-29 and HCT-116 colon cancer cells. a Viability of HT-29 and HCT-116 colon cancer cells following exposure to serum-free medium was measured using the MTT assay. b Small-EVs (10 μg protein) from HT-29 and HCT-116 cells were subjected to immunoblotting with antibodies against small-EV marker proteins, namely CD63 and Alix. c, d Small-EVs derived from HT-29 and HCT-116 cells were analyzed by transmission electron microscopy. All small-EV samples contained vesicles of variable sizes in the range of 70–200 nm. Scale bar, 100 nm. e, f Size distribution of small-EVs derived from two different colon cancer cell lines was measured using nanoparticle tracking analysis. The images shown are representative and the size of HT-29- and HCT-116-derived small-EVs is 184.2 ± 46.5 and 178.5 ± 44.4 nm, respectively (n = 3)