Fig. 5

dBP4 inhibits angiogenesis in 4T1.2luc tumours. 4T1.2luc cells (5 × 10⁴) were implanted into the mammary fat pad of female BALB/c mice (n = 3/group). Once tumours reached a MTD of 8–8.5 mm, mice received an intra-tumour injection of 50 μg dBP4 or PBS every 2–3 days. Once tumours reached a MTD of 17 mm, mice were sacrificed. Primary tumours were excised and blood vessels visualized by staining for CD31 a shows representative images of CD31+ stained tumours from PBS or dBP4 treated mice. IC: Negative isotype control. (scale bar 50 μm). Enlarged area shows vessel morphology. b Mean ± SEM CD31+ vessels in 5 fields of view/tumour (n = 3/group). (*P < 0.05, unpaired Students t-test)