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Fig. 4 | BMC Cancer

Fig. 4

From: The oncogene KRAS promotes cancer cell dissemination by stabilizing spheroid formation via the MEK pathway

Fig. 4

Comparison of cDNA microRNA data between ID8-2D and ID8-3D, and ID8-KRAS-2D and ID8-KRAS-3D cells, respectively. a Comparison of expression plot analysis of ID8-2D and ID8-3D cells with ID8-KRAS-2D and ID8-KRAS-3D cells, respectively. b Number of differentially expressed genes in the comparative microarray analyses. Fold change cut-off: upregulated > 2-fold or downregulated < 2-fold. c The Venn diagram shows the degree of intersection between modulated genes in ID8-2D and ID8-3D cells with ID8-KRAS-2D and ID8-KRAS-3D cells. d Expression of the genes upregulated in ID8-KRAS-3D cells. Expression mRNA levels of top 5 genes upregulated only in ID8-3D KRAS cells were evaluated by RT-qPCR. The expression of each gene level was normalized using PPIA mRNA as the internal standard. Expression levels were calculated by the comparative Ct method using PPIA as an endogenous reference gene. Error bars represent the mean ± SEM (* p < 0.05)

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