Fig. 3

Proliferation of ID8 and ID8-KRAS cells in vivo. Mice were i.p. injected with EdU after 48 h of cancer cell inoculation. After 2 h of EdU administration, 8 ml of normal saline was i.p. injected into mice, and cells were recovered from the peritoneal cavity using peritoneal washes. To detect EdU incorporation, EdU was labeled with Alexa Fluor 647. a Comparison of the EdU uptake of ID8-GFP and ID8-KRAS-GFP cells from peritoneal washes. EdU-stained cells were analyzed by flow cytometry. The area of EdU-positive cells in GFP-positive cells was shown by the dotted frame, and that of GFP-positive cancer cells was shown by the solid frame. b A quantitative analysis of the EdU-positive cells in the GFP-positive cancer cells. The values shown represent the mean ± SEM (* p < 0.05, n = 6 mice per group). The proportion of proliferating cells (%) = 100 × (Number of EdU-positive cells in the cancer cell area) / (Number of all cells in the cancer cell area)