The hypermethylation of p16 gene exon 1 and exon 2: potential biomarkers for colorectal cancer and are associated with cancer pathological staging

Table 1 The primer sequences and PCR conditions

Primer name	Forward primers^a (5′-3′)	Reverse primers^a(5′-3′)	Anneal temp.	Product
p16 exon 1
Outer	TTAGAGGATTTGAGGGATAGGGT	TACAAACCCTCTACCCACCTAAAT	56 °C	324 bp
Inner	GGATTTGAGGGATAGGGT	CCCTCTACCCACCTAAAT	56 °C	313 bp
p16 exon 2
Outer	TGGTAGGTTATGATGATGGGTAG	ATCCTCACCTAAAAAACCTTCC	54 °C	321 bp
Inner	GGTTATGATGATGGGTAG	TTACTACCTCTAATACCCC	53 °C	273 bp

^aSequence differences between modified primers according bisulfite-converted DNA and unconverted DNA are indicated in boldface type

ISSN: 1471-2407