Table 4 Details of antibodies used in the IHC-protocols of the current study
Antibody | Host species | Catalog No. | Clonality | Dilution | Manufacturer/distributor |
|---|---|---|---|---|---|
Anti-Human HER3 | Mouse | M7297 | DAK-H3-IC | 1:100 | DAKO A/S, Glostrup, Denmark |
FLRF/RNF41 Antibody | Rabbit | A300-049A | polyclonal | 1:3000 | Bethyl Laboratories, Inc., Montgomery, Texas, USA |
Anti-Nedd4, WW2 domain | Rabbit | #07–049 | polyclonal | 1:750 | Merck KGaA, Darmstadt, Germany |
Cytokeratin 5 Antibody | Mouse | NCL-L-CK5 | XM26 | 1:150 | Leica Biosystems Newcastle Ltd., Newcastle Upon Tyne, UK |
Cytokeratin 14 Antibody | Mouse | NCL-L-LL0022 | LL0022 | 1:150 | Leica Biosystems, Newcastle Ltd., Newcastle Upon Tyne, UK |
Anti-human Ki67 | Mouse | BSH-7302 | BS4 | 1:100 | Nordic BioSite AB, Täby, Sweden |