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Table 4 Associations of overall survival and recurrence free survivals with DNA methylation age stratified by menopause and clinicopathological features

From: Decelerated DNA methylation age predicts poor prognosis of breast cancer

Stratified variables DNAm age (quartile)a Survival Recurrence
N HRb (95% CI) N HRb (95% CI)
Menopause Status
 Pre First 80 1.00 (ref) 72 1.00 (ref)
Second-Fourth 149 0.87 (0.30–2.58) 133 1.16 (0.42–3.21)
 Post First 153 1.00 (ref) 117 1.00 (ref)
Second-Fourth 583 0.40 (0.24–0.69) 486 0.58 (0.29–1.16)
 P for interaction 0.3761   0.963
Clinical stage
 Stage I&II First 136 1.00 (ref) 103 1.00 (ref)
Second-Fourth 432 1.24 (0.62–2.49) 341 0.80 (0.32–1.96)
 Stage III&IV First 56 1.00 (ref) 39 1.00 (ref)
Second-Fourth 131 0.39 (0.17–0.91) 97 1.07 (0.39–2.92)
 P for interaction 0.159   0.665
ER status
 negative First 99 1.00 (ref) 77 1.00 (ref)
Second-Fourth 79 0.82 (0.31–2.15) 61 0.60 (0.19–1.84)
 positive First 95 1.00 (ref) 67 1.00 (ref)
Second-Fourth 493 0.54 (0.29–0.99) 385 0.77 (0.33–1.78)
 P for interaction 0.092   0.9972
HER2 status
 negative First 156 1.00 (ref) 119 1.00 (ref)
Second-Fourth 488 0.92 (0.51–1.64) 382 1.14 (0.57–2.31)
 Equivocal /positive First 37 1.00 (ref) 25 1.00 (ref)
Second-Fourth 81 0.37 (0.09–1.58) 63 0.18 (0.03–1.21)
 P for interaction 0.217   0.133
PAM50
 Luminal A First 25 1.00 (ref) 13 1.00 (ref)
Second-Fourth 204 0.72 (0.17–2.97) 149 0.59 (0.11–3.24)
 Luminal B First 22 1.00 (ref) 16 1.00 (ref)
Second-Fourth 100 0.65 (0.21–2.06) 73 0.29 (0.06–1.46)
 HER2-enriched&Basal-like First 84 1.00 (ref) 61 1.00 (ref)
Second-Fourth 71 1.91 (0.67–5.41) 48 0.66 (0.17–2.53)
 P for interaction 0.016   0.966
  1. a Patients were divided into quartiles according to DNAm age. First quartile: 2.2–37.6; Second quartile: 37.7–49.8; Third quartile: 49.9–64.9; Fourth quartile: 65–157
  2. b Adjusted for chronological age (continuous), race, clinical stage, menopause Status, ER status, HER2 status and PAM50 subtype. When one of the confounders was the variable for stratifying, it was not adjusted in the model
  3. Significant ones are in boldface