Decelerated DNA methylation age predicts poor prognosis of breast cancer

BMC Cancer

Table 3 Association of recurrence free survivals with DNA methylation age

DNAm Age	N	Overall recurrence free survival			Five-year recurrence free survival
DNAm Age	N	HR (95% CI) ^a	HR (95% CI) ^b	HR (95% CI) ^c	HR (95% CI) ^a	HR (95% CI) ^b	HR (95% CI) ^c
Continuous
(Per ten year)	889	0.89 (0.81–0.98)	0.86 (0.77–0.96)	0.86 (0.76–0.97)	0.87 (0.78–0.98)	0.84 (0.75–0.95)	0.86 (0.75–0.98)
Categorical (quartile) ^d
First	215	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)
Second	230	0.82 (0.48–1.39)	0.76 (0.44–1.31)	0.77 (0.43–1.36)	0.77 (0.43–1.36)	0.70 (0.39–1.25)	0.71 (0.39–1.31)
Third	227	0.82 (0.49–1.37)	0.72 (0.42–1.23)	0.70 (0.39–1.29)	0.76 (0.43–1.36)	0.65 (0.36–1.17)	0.68 (0.36–1.32)
Fourth	217	0.48 (0.25–0.90)	0.40 (0.21–0.78)	0.39 (0.19–0.80)	0.39 (0.19–0.80)	0.31 (0.15–0.66)	0.32 (0.14–0.74)
P for trend		0.031	0.009	0.014	0.014	0.003	0.020
Categorical (First quartile and second-fourth quartile)
First	215	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)
Second-Fourth	674	0.71 (0.46–1.08)	0.64 (0.41–1.00)	0.66 (0.39–1.09)	0.64 (0.40–1.02)	0.57 (0.35–0.93)	0.62 (0.35–1.07)

^aNo adjustment
^bAdjusted only for chronological age (continuous)
^cAdjusted for chronological age (continuous), race, clinical stage, Menopause Status, ER status, HER2 status and PAM50 subtype
^dPatients were divided into quartiles according to DNAm age. First quartile: 2.2–37.6; Second quartile: 37.7–49.8; Third quartile: 49.9–64.9; Fourth quartile: 65–157
Significant ones are in boldface

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