Decelerated DNA methylation age predicts poor prognosis of breast cancer

BMC Cancer

Table 2 Association of overall and five-year survival with DNA methylation age

DNAm Age	N	Overall survival			Five-year survival
DNAm Age	N	HR (95% CI) ^a	HR (95% CI) ^b	HR (95% CI) ^c	HR (95% CI) ^a	HR (95% CI) ^b	HR (95% CI) ^c
Continuous
(Per ten year)	1076	1.01 (0.94–1.08)	0.92 (0.85–1.00)	0.94 (0.86–1.02)	0.92 (0.84–1.01)	0.84 (0.75–0.93)	0.85 (0.76–0.96)
Categorical (quartile) ^d
First	269	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)
Second	269	0.80 (0.51–1.26)	0.66 (0.42–1.05)	0.64 (0.39–1.04)	0.59 (0.34–1.03)	0.49 (0.28–0.85)	0.52 (0.29–0.92)
Third	269	0.79 (0.51–1.24)	0.56 (0.35–0.89)	0.56 (0.34–0.93)	0.66 (0.39–1.12)	0.46 (0.27–0.78)	0.49 (0.27–0.87)
Fourth	269	1.00 (0.66–1.53)	0.56 (0.35–0.90)	0.60 (0.36–1.04)	0.60 (0.34–1.03)	0.33 (0.18–0.59)	0.38 (0.20–0.72)
P for trend		0.939	0.014	0.065	0.075	0.001	0.004
Categorical (First quartile and second-fourth quartile)
First	269	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)	1.00 (Ref)
Second-Fourth	807	0.86 (0.61–1.22)	0.59 (0.41–0.86)	0.60 (0.40–0.91)	0.61 (0.41–0.93)	0.42 (0.27–0.65)	0.47 (0.29–0.76)

^aNo adjustment
^bAdjusted only for chronological age (continuous)
^cAdjusted for chronological age (continuous), race, clinical stage, menopause status, ER status, HER2 status and PAM50 subtype
^dPatients were divided into quartiles according to DNAm age. First quartile: 2.2–37.6; Second quartile: 37.7–49.8; Third quartile: 49.9–64.9; Fourth quartile: 65–157
Significant ones are in boldface

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