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Fig. 4 | BMC Cancer

Fig. 4

From: Krüppel-like factor 9 and histone deacetylase inhibitors synergistically induce cell death in glioblastoma stem-like cells

Fig. 4

Forced KLF9 expression and HDAC inhibitor LBH589 induced apoptosis. a, b Early stage apoptosis (Annexin-V positive, PI negative) and late stage apoptosis or necrosis (Annexin-V positive, PI positive) in GBM1A cells (A) under different treatment for 24 h were measured by flow cytometry. Similar experiments were performed in GBM1B cells (B). c Increased cleaved caspase 3 and cleaved PARP were detected in GSCs treated with Dox + LBH589. d Expression of pro-apoptotic proteins was examined in GSCs. Pro-apoptotic proteins Bak, Bik, Bax, Bid and Noxa were up-regulated by KLF9 expression. e Expression of anti-apoptotic proteins survivin and XIAP was down regulated by LBH589 alone or in the presence of Dox. f The pan-caspase inhibitor z-vad had minimal protective effect on KLF9-expressing GSCs treated with LBH589. *: P < 0.05

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