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Fig. 3 | BMC Cancer

Fig. 3

From: Krüppel-like factor 9 and histone deacetylase inhibitors synergistically induce cell death in glioblastoma stem-like cells

Fig. 3

HDAC inhibitor LBH589 induced cell cycle arrest but not mitotic catastrophe. a Cell cycle analysis of GBM1A cells treated with Dox, LBH589, and Dox + LBH589 for 24 h. Cells were not synchronized before experiments. In the combined treatment of LBH589 and Dox, cell cycle was arrested at S-phase (6.57%) and there was a significant increase in sub-G1/G0 portion (~ 19%), indicating cell death. b Similar cell cycle experiment results were found in GBM1B neurosphere cells. c Expression of cell cycle modulators including cyclins, p21, p27 and cdc2 were examined in GBM1A cells treated with Dox and LBH589 alone or combined. d Time course of cell cyle progression under the treatment of Dox + LBH589. Cells were growth arrested by thymidine for 8 h followed by release from cell cycle arrest for indicated time points. Cell cycle analysis indicated that at 18 h, Dox + LBH589-treated cells had a slight decrease at G2/M phase without increase in cell accumulation at sub-G1/G0 phase. At 30 h and 42 h, there was a dramatic decrease in cells at G2/M phase and a slight increase in cells at sub G1/G0 phase, indicating no accumulation of cells in G2/M phase before cell death. *: P < 0.05

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