Fig. 2
Isobologram analysis indicated KLF9 expression and HDAC inhibitors synergistically induced GSC death. a, b Enhanced cell viability loss induced by KLF9 expression and HDAC inhibitors SAHA and TSA in GBM1A (a) and GBM1B cells (b). MTS assays demonstrated that SAHA (10 μmol/L) or TSA (50 nmol/L) alone did not markedly induce cell death. The combination of Dox and SAHA or TSA induced ~ 60–65% GSC loss after 48 h of simultaneous treatment. c Systematic characterization of cell viability (control as 100%) in GSCs treated with LBH589 alone at different concentrations, or in the presence of Dox (0.1 μg/ml). IC50 was calculated by Graphpad Prism. d Cell viability assays (control as 100%) under the treatment of Dox at different concentrations, or in the presence of LBH589 (25 nmol/L). Isobologram index (Ix) was calculated as 0.41, indicating synergistic cell killing effect of these two treatments. *: P < 0.05