Fig. 2

L861Q TaqMan assay (droplet digital PCR). 2D plots and amounts of L861Q-positive droplets (indicated in red) obtained via ddPCR with FFPE DNA (a) or cfDNA (b to h). a) the primary tumour, b) on the day of surgery (D0), c) before concomitant chemotherapy (CT) and radiotherapy (RT) (pre-RT), d) 1 month after RT (post-RT), e) 1 month after the first cycle of CT (CT-1), f) 1 month after four cycles of CT (CT-4), g) after 9 weeks of afatinib treatment (post-TKI), and h) after two cycles of lomustine with bevacizumab (Lom + Bev-2). The plasma DNA concentration is expressed as the amount per ml of plasma. ddPCR, droplet digital PCR; FFPE, formalin-fixed, paraffin-embedded; pDNA, plasma DNA; AU, arbitrary units; WT, wild type; CT, chemotherapy; RT, radiotherapy