Fig. 6

β-cateninS45Y and ∆N90-β-catenin mutants activate TCF/LEF reporter in mouse HCC cell lines. a Western blot analysis confirmed the expression of transfected β-cateninS45Y or ∆N90-β-catenin plasmids in HCC3–4 and HCC4–4 mouse HCC cell lines. b TCF/LEF reporter activity in HCC3–4 and HCC4–4 mouse HCC cell lines following the transfection of pT3-EF1α-β-cateninS45Y and pT3-EF1α-∆N90-β-catenin, as analyzed using TopFlash/FopFlash reporter assays. Student’s t-test: ***P < 0.001