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Fig. 3 | BMC Cancer

Fig. 3

From: Uc.416 + A promotes epithelial-to-mesenchymal transition through miR-153 in renal cell carcinoma

Fig. 3

Interaction between Uc.416 + A and miR-153. a The results of qRT-PCR analysis for the expression of miR-153 in 32 renal cell carcinoma (RCC) tissues and corresponding normal kidney tissues (N). Statistical differences were evaluated with the paired T test. b The correlation between Uc.416 + A and miR-153 in RCC tissues. Spearman correlation coefficient and P-values are indicated. c qRT-PCR analysis for the expression of miR-153 in 786-O cells transfected with negative control or two different siRNAs. d qRT-PCR analysis for the expression of miR-153 in 786-O cells transfected with negative control or miR-153 mimics. The results are expressed as the mean and S.D. of triplicate measurements. ***P < 0.001. e qRT-PCR analysis for the expression of Uc.416 + A in 786-O cells transfected with negative control or miR-153 mimics. The results are expressed as the mean and S.D. of triplicate measurements. ***P < 0.001. f The luciferase activity of 786-O cells co-transfected with a control pGL3-Promoter vecor containing a wild type Uc.416 + A sequence or a pGL3-Promoter vector containing a mutated Uc.416 + A sequence. The results are expressed as the mean and S.D. of triplicate measurements. ***P < 0.001. N.S., not significant; WT: wild type; Mut: sequence with a mutated miR-153 binding site

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