Fig. 4

a Western blot and densitometry for c-FLIP long in SKBR3-Par and SKBR3-L cells. C-FLIP short was not detectable by Western blotting in either SKBR3-Par or SKBR3-L cells. b The effect of 8-h of TRAIL treatment (100 ng/ml) on caspase 8 and caspase 9 activity normalised to cell viability, relative to untreated controls in SKBR3-Par and SKBR3-L cells. c FOXO3a qRT-PCR in SKBR3-Par and SKBR3-L cells. d Western blot and densitometry for p-AKT (s473) relative to total AKT in SKBR3-Par and lapatinib resistant cell lines. e Percentage growth inhibition by LY294002 (LY) and/or TRAIL (TR) (72 h) in SKBR3-Par cells. Error bars represent the standard deviation of triplicate independent experiments. ‘*’ indicates a p value of < 0.05 as calculated by Student’s t-test