Fig. 3

Androgen-dependent transcriptional responses of prostate cancer cell lines. a RT-qPCR shows androgen treatment (2 nM R1881, 12 h) induces FKBP5, ABCC4, EAF2, and PIAS1 expression in PC3-AR cells. b Eigengene expression plots from WGCNA of PC3-AR RNA-sequencing. c Venn diagram of all differentially expressed transcripts in PC3-AR, LNCaP, and VCaP cell lines after 2 nM R1881 androgen treatment. Differential fold change was set to the following parameters: > 1.5-fold or < 0.67-fold; adj. p ≤ 0.01. LNCaP and VCaP treatments were conducted for 24 h while PC3-AR data was taken from a 12 h treatment. d Heatmap displaying normalized enrichment scores (NES) generated from a pre-ranked Gene Set Enrichment Analysis (GSEA) of androgen-treated prostate cancer cell lines. Standard GSEA methods were limited to the MSigDB hallmarks. e Principal component analysis (PCA) plot of mRNA expression data generated from RNA-sequencing. Data derived from LNCaP, VCaP, and PC3-AR cells before and after androgen treatment