Fig. 1

Characterization of PC3 cells stably transduced with WT AR. a AR protein expression level in PC3-AR cells compared to LNCaP and VCaP cells. b Immunofluorescence localization of AR in PC3-AR cells before and after treatment with synthetic androgen (2 nM R1881, 15 min), which induces nuclear import of AR. c AR complexes isolated by immunoprecipitation and examined for Hsp90β and Hsp70 content, which is released by androgen. d Immunoblot showing androgen-induced expression of FKBP51 in LNCaP and PC3-AR cells after an overnight treatment of 2 nM R1881. e Androgen-induced G1 cell cycle arrest after a 12 h R1881 treatment