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Fig. 2 | BMC Cancer

Fig. 2

From: Post-transcriptional regulation of cancer/testis antigen MAGEC2 expression by TRIM28 in tumor cells

Fig. 2

Knockdown of TRIM28 reduces MAGEC2 protein level and does not affect MAGEC2 mRNA level in melanoma cells. a, b Knockdown of TRIM28 decreases MAGEC2 protein level in A375 and Hs 695 T cells. TRIM28-specific siRNAs or control siRNA (si-NC) were transfected into A375 (a) or Hs 695 T cells (b) for 48 h, and cell lysates were immunoblotted with anti-MAGEC2 or anti-TRIM28 antibodies. Expression levels of GAPDH are indicated as an internal control. c, d Knockdown of TRIM28 does not affect the expression levels of other members of MAGE family in A375 cells (c) or Hs 695 T cells (d). A375 or Hs 695 T cells were transfected with TRIM28-specific siRNA for 48 h, and cell lysates were immunoblotted with indicated antibodies. e, f, g, h Knockdown of TRIM28 does not affect MAGEC2 mRNA level in A375 (e, f) or Hs 695 T cells (g, h). e, f Expression of MAGEC2 mRNA was examined in TRIM28-specific siRNA transfected-A375 cells by conventional PCR (E) or quantitative real-time PCR (f). g, h Knockdown of TRIM28 does not affect MAGEC2 mRNA level in Hs 695 T cells. Expression of MAGEC2 mRNA was examined in TRIM28-specific siRNA transfected-Hs 695 T cells by conventional PCR (g) or quantitative real-time PCR (h). Data are represented as mean ± SD. Each experiment was repeated three times

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