Fig. 4

Fluorescence microscopy of γH2A.X. IGROV1 cells were incubated with 2.5 MBq ¹⁷⁷Lu-DOTA-chCE7 for 4 h or with 100 nM MK1775 for 48 h. For combination, cells were simultaneously incubated with both agents. After 4 h incubation, ¹⁷⁷Lu-DOTA-chCE7 was removed and MK1775 was further incubated until 48 h were reached. Cells were fixed and stained and γH2A.X foci were counted and cells grouped depending on (a) no observed (b) ≤ 5 (c) ≥ 6 or (d) non-distinguishable γH2A.X foci (intensively positive). a-d shows examples of IGROV1 cells with accordant numbers of γH2A.X foci. (e) summarizes all γH2A.X positive cells expressed in percent of total cell numbers counted