Fig. 1

Knockdown of ANRIL functions in HepG2 cells proliferation and apoptosis. HepG2 cells were transfected with the expression vectors of sh-ANRIL#1 and sh-ANRIL#2 to knockdown ANRIL expression. a qRT-PCR assay was used for indicating the relative expression level of ANRIL in these transfected cells. b Cell viability was examined in HepG2 cells after transfection with sh-ANRIL#1 and sh-ANRIL#2 by CCK-8 assay. c and d Protein levels of CyclinD1, p53 and p21 in these transfected cells were detected by western blot assay. e Cell apoptosis and f the protein levels of pro-Caspase-3/− 9 and cleaved-Caspase-3/− 9 were respectively determined by flow cytometry and western blot. ANRIL: CDKN2B antisense RNA 1; qRT-PCR: quantitative real-time reverse-transcription polymerase chain reaction; CCK-8: Cell Counting Kit-8; *P < 0.05; **P < 0.01, ***P < 0.001