Fig. 6
PPARγand PTEN are increased in HeLa cells with I2 treatment. Monolayer and sphere cultures of HeLa cells were treated with 200 μM (I2) for 24 h and PPARγ protein was quantified by Western blot and densitometry is reported as percent change with respect to control without treatment. (a, b). PTEN expression was analyzed by qPCR and normalized to GAPDH expression (c). HPV18 E6 and E7 oncoproteins expression were analyzed by qPCR and normalized to GAPDH expression (d, e). Data are expressed as mean ± SD (n = 3 independent assays), and the asterisk indicates a significant difference with respect to the control without treatment. (*P < 0.05, **P < 0.01)