Fig. 4

EN2 protein expression in human ovarian tissue conveys poor prognosis. Enzymatic immunohistochemistry examples of normal kidney (a), normal ovary with surface epithelium (b) and ovarian adenocarcinoma stained for EN2 (c, e, g), or adjacent sections stained for H&E (d, f, h), at 10× magnification. The assigned staining intensity (1–3+) is indicated. EN2 staining (brown) is present in the cytoplasm, but not in the nucleus, and is not seen in the surrounding stroma. On the H&E stained sections, pink staining represents cytoplasm and blue staining represents the nucleus. (i) Survival curves for EN2 negative versus EN2 positive protein expression in high-grade serous ovarian tumours are shown (EN2 -ve = 0–4 IHC score; EN2 + ve = 5–12 IHC score). The median survival was significantly lower for all HGSOCs with EN2 + ve staining (10 months) versus those with EN2 –ve staining (17.5 months) (p = 0.0103)