Fig. 4

ASCs affect tumor growth. a Proliferation of 4 T1 cells in the presence of vASC P3 or ASC.B6 at a ratio of 2.5:1, separated in Transwell inserts. The bars show the mean ± SD from 3 independent experiments, the statistical analysis was t-test with P-values set at: **P < 0.01. b IGF1 protein expression was analyzed with Western blotting in total cell lysates and conditioned media of vASCs harvested at P3 or P6 and ASC.B6 cells. The IGF1 signals from cell lysates were normalized to β-actin, and then divided with the value of vASCs at P3. The picture shows a representative of at least 3 independent experiments. c Neutralizing antibody against IGF1 (2 μg/ml) inhibited the proliferation of 4 T1 cells in the presence of ASC.B6 in Transwell inserts. The bars show the mean ± SD from 3 independent experiments, the statistical analysis was t-test with P-values set at: *P < 0.05