Table 1 Primer sequences and thermal profiles
From: Contribution of KCTD12 to esophageal squamous cell carcinoma
Genes | Sequence (5’to 3’) | Amplification Size(bp) |
|---|---|---|
GAPDH | F: GGAAGGTGAAGGTCGGAGTCA | 101 bp |
R:GTCATTGATGGCAACAATATCCACT | ||
TWIST1 | F: GGAGTCCGCAGTCTTACGAG | 201 bp |
R:TCTGGAGGACCTGGTAGAGG | ||
BMI1 | F: CGTGTATTGTTCGTTACCTGGAGAC | 204 bp |
R:CATTGGCAGCATCAGCAGAAGG | ||
Cripto | F: GGGATACAGCACAGTAAGGAG | 295 bp |
R:ACGGTGGTAGTTGTCGAGTC | ||
KLF4 | F:TCTTCTCTTCGTTGACTTTG | 210 bp |
R:GCCAGCGGTTATTCGG | ||
Nanog | F: GGCAATGGTGTGACGCAGAAGGC | 137 bp |
R:GCTCCAGGTTGAATTGTTCCAGGTC | ||
MSI1 | F: TGAGCAGTTTGGGAAGGTG | 117 bp |
R: TCACACACTTTCTCCACGATG | ||
MEIS1 | F: ATGACACGGCATCTACTCGTTC | 105 bp |
R: TGTCCAAGCCATCACCTTGCT | ||
SOX2 | F: AACAGCCCGGACCGCGTCAA | 189 bp |
R: TCGCAGCCGCTTAGCCTCGT | ||
MAML1 | F: TCTCGCGGAACAGGAGA | 123 bp |
R: GCAGCAGAGGACCCTGTG | ||
PYGO2 | F: GTCCCCCACTCCATGGCCGCCTCG | 147 bp |
R: TCGCTTCTTTTCTGGACTCTTC | ||
EGFR | F: ACCGGCAGGATGTGGAGATC | 186 bp |
R: GGCCGACAGCTATGAGATGGA | ||
ABCG2 | F: TGAGGGTTTGGAACTGTGG | 155 bp |
R: GATTCTGACGCACACCTGG | ||
ABCC4 | F: GAAATTGGACTTCACGATTTAAGG | 125 bp |
R: TTCCACAGTTCCTCATCCGT | ||
DIDO1 | F: TTTGTTGGTCCAGTTTCGCCTTC | 220 bp |
R: ACGACAAGCAAGAGACTGTTTCAC | ||
SMARCA4 | F: TGTGAGGAGGAGGAGGAGAA | 164 bp |
R: CGCTTCCGTGATGATTTCTT |