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Fig. 1 | BMC Cancer

Fig. 1

From: Expression of oxidized protein tyrosine phosphatase and γH2AX predicts poor survival of gastric carcinoma patients

Fig. 1

The expression of Ox-PTP and γH2AX in human gastric carcinomas, and statistical analysis. a Western blotting for Ox-PTP and γH2AX to validate antibodies. The expression of Ox-PTP and γH2AX increased with treatment of 400 μM H2O2 for 24 h in NCI-N87, MKN45, and KATO-III gastric cancer cells. b Ox-PTP is expressed in both the cytoplasm and the nuclei of tumor cells, and the expression of γH2AX is observed in the nuclei of tumor cells. Original magnification, × 400. c Receiver operating characteristic curve analysis for the estimation of cut-off points for the staining scores of Ox-PTP and γH2AX. The highest positive cut-off points for the nuclear expression of Ox-PTP (arrow head), cytoplasmic expression of Ox-PTP (black arrow), and γH2AX (empty arrow) were estimated at the highest area under the curve (AUC) value for the estimation of death and survival events of patients. The likelihood points for the immunohistochemical staining score of nuclear Ox-PTP, cytoplasmic Ox-PTP, and γH2AX expression were 6, 6, and 5, respectively

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